Scientific Literature on Nanotechnology

In-depth immunophenotyping reveals significant alteration of lymphocytes in buffalo with brucellosis

The aims of the study were to (i) verify the cross-reactivity of 16 monoclonal antibodies (mAbs) developed against human, bovine, and ovine antigens; (ii) evaluate lymphocyte subset alterations in brucellosis positive buffalo; (iii) evaluate the use of the canonical discriminant analysis (CDA), with flow cytometric data, to discriminate BRC positive from negative animals.

Camouflaged Fluorescent Silica Nanoparticles Target Aggregates and Condensates of the Amyloidogenic Protein Tau

Nanoparticles (NPs) offer significant potential for probing the mechanisms of protein fibrillation and may be capable of redirecting conformational transitions. Here, we camouflaged dye-doped silica NPs through functionalization with tau molecules to impart them the ability to associate with protein assemblies such as aggregates or condensates.

Fluorescent Silica Nanoparticles Targeting Mitochondria: Trafficking in Myeloid Cells and Application as Doxorubicin Delivery System in Breast Cancer Cells

Fluorescent silica nanoparticles (SiNPs) appear to be a promising imaging platform, showing a specific subcellular localization. In the present study, has been investigated their preferential mitochondrial targeting in myeloid cells.

CNA Profiling of Single CTCs in Locally Advanced Esophageal Cancer Patients during Therapy Highlights Unexplored Molecular Pathways

In the present work, is described the evolution of circulating tumor cells (CTCs) released into the bloodstreams of 11 patients affected by locally advanced esophageal cancer (EC) during clinical treatments.

Uptake and Intracellular Trafficking Studies of Multiple Dye-Doped Core-Shell Silica Nanoparticles in Lymphoid and Myeloid Cells

Since most biologically active macromolecules are natural nanostructures, operating in the same scale of biomolecules gives the great advantage to enhance the interaction with cellular components. In this in vitro study, is reported a physicochemical characterisation of fluorescent silica nanoparticles, interacting with biological models, describing the specific triggered biologic response.

What information could the main actors of liquid biopsy provide? —a representative case of non-small cell lung cancer (NSCLC)

In this study is provided the proof-of-concept that the complementary use of ctDNA and ctcDNA represents a reliable, minimally invasive and dynamic tool for a more comprehensive view of tumor evolution.

EpCAMhigh and EpCAMlow circulating tumor cells in metastatic prostate and breast cancer patients

Blood samples of 108 castration-resistant prostate cancer patients and 22 metastatic breast cancer patients were processed, using protocols and tools developed in the CTC-Trap program to investigate the presence of EpCAMhigh and EpCAMlow CTCs.

Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap)

In this study are presented the results obtained from 34 metastatic cancer patients subjected to Diagnostic LeukApheresis to determine tumor heterogeneity.

Targeted Delivery of Neutralizing Anti-C5 Antibody to Renal Endothelium Prevents Complement-Dependent Tissue Damage

Complement activation is largely implicated in the pathogenesis of several clinical conditions and its therapeutic neutralization has proven effective in preventing tissue and organ damage.A problem that still needs to be solved in the therapeutic control of complement-mediated diseases is how to avoid side effects associated with chronic neutralization of the complement system, in particular, the increased risk of infections. In this study this issue was addressed developing a strategy based on the preferential delivery of a C5 complement inhibitor to the organ involved in the pathologic process.

Multiple Dye Doped Core-Shell Silica Nanoparticles: Outstanding Stability and Signal Intensity Exploiting FRET Phenomenon for Biomedical Applications

Here is presented a one-pot synthesis of core-shell silica nanoparticles (SiNPs) as a novel fluorescent probe for biological applications. SiNPs were doped with a different number of dyes to ensure high efficiency Fluorescence Resonance Energy Transfer (FRET).