Specialty school thesis in Clinical Biochemistry title:
“Effects of pharmacologically active drugs doped silica nanoparticles on breast cancer human cells”
University of Bari “Aldo Moro” – Faculty of Medicine and Surgery
Clinical Biochemistry Specialty School – Analytical and Technical Specialization
Candidate: Dr. Giancarlo Sciortino
Supervisor: Prof. Ferdinando Capuano
Assistant supervisors: Dr. Alfredo Ventola, Dr. Amalia Azzariti
The aim of this work was to evaluate in vitro the effects of silica nanoparticles on breast cancer human cells, analyzing:
- Citotoxicity assays on human tumor cell lines of AcZon nanoparticles, either empty, either doped with the active drug inside the core;
- Rate and extent of intracellular drug accumulation;
- Effects of exposure to doxorubicin and doxorubicin doped nanoparticles on cells life cycle;
- SAXS spectra of cells and nanoparticles.
From the results, it is clear that the cytotoxic action of the antineoplastic drug doxorubicin when doped in nanoparticles is improved, because of:
- Higher concentration of the drug inside tumor cells;
- Reduced ability of tumor cells to eliminate the drug doped in nanoparticles.