AcZon NanoTandem line has been created to answer to cytometrists’ need of a new class of tandem dyes stable across the time and not requiring a frequent resetting of the instruments. AcZon NanoTandems, on a par with conventional tandem dyes, exploit the FRET (Fluorescence Resonance Energy Transfer) phenomenon which allows to achieve a larger Stokes shift thanks to a fluorophores chain (from 2 to 6 different fluorophores) acting among them as donors and acceptors.
The silica shield protects all the molecules, mainly the most sensitive, from the interferences of the external environment allowing the achievement of satisfactory results with a unique setting of instruments parameter.
Nanoparticles size arranges fluorophores at the optimal distance to obtain the maximum efficacy of the energy transfer, increasing the emission of the last acceptor and limiting the unwilled emission of the intermediate fluorophores of the chain.
The list of the NanoTandems available for sale or for custom conjugation is available following this link.